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Private company

FlexGen

Logo: Joris Parmentier, Public domain, via Wikimedia Commons3

Biotechnology company

FlexGen was a biotechnology company based in Leiden, Netherlands. FlexGen was a spin-off from Leiden University Medical Centre and Dutch Space (part of EADS) and had proprietary technologies for laser based in-situ synthesis of oligonucleotides and other biomolecules. On 21 December 2015, Flexgen Bv in Leiden (South Holland) was declared bankrupt by the court in Gelderland Source.2 Wikipedia text CC BY-SA 4.0

Last updated Oct 21, 2026.

Overview

2004Founded1
  • Industrybiotechnology1
  • HeadquartersLeiden1
  • Websitewww.flexgen.nl
  • Legal formbesloten vennootschap1

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FlexGen was a biotechnology company based in Leiden, Netherlands. FlexGen was a spin-off from Leiden University Medical Centre and Dutch Space (part of EADS) and had proprietary technologies for laser based in-situ synthesis of oligonucleotides and other biomolecules. On 21 December 2015, Flexgen Bv in Leiden (South Holland) was declared bankrupt by the court in Gelderland Source.2

Products and brands

Products

FleXelect

FleXelect oligopools Archived 2011-07-24 at the Wayback Machine consist of custom oligonucleotides in solution and can be used for in solution target enrichment prior to next generation DNA sequencing. Target enrichment or In solution hybrid selection is a method for genomic selection in an increasing number of applications such as;

Analysis of custom genomic regions of interest (e.g. specific genes, multiple variants and/or complete pathways).

Analysis of Chromosomal translocation

Validation of Single-nucleotide polymorphism or SNPs (typically after whole genome or whole exome studies)

Read the full text

Other research and diagnostic applications (e.g. Synthetic biology)

An example of a recent application is testing of the BRCA1 and BRCA2 breast cancer risk genes

FlexArrayer

The FlexArrayer Archived 2011-07-24 at the Wayback Machine is an in-house custom oligonucleotide synthesis instrument. The FlexArrayer facilitates high throughput synthesis of FleXelect oligopools for in-solution target enrichment as well as custom microarray production. The FlexArrayer is also applicable for array based re-sequencing.

The FlexArrayer provides microarray and oligopool synthesis typically used by:

Genomics centres and sequencing facilities

Health and safety institutes & microbiology labs

Technology innovators in the fields of: Surface chemistries, PNA's (Peptide nucleic acid), siRNA's (Small interfering RNA) and more

Technology

Production of microarrays and FleXelect oligopools is done with the FlexArrayer (see image) using proprietary technology. The FlexArrayer synthesizes custom probesets on a substrate based on oligonucleotide deprotection technology;

Before the first oligonucleotide synthesis step the complete DNA microarray surface is covered by photolabile groups.

Those spots the first nucleotide addition is to occur are individually activated by the laser.

The nucleotide solution is washed over the microarray surface and the nucleotides chemically bind to the activated spots.

All nucleotides contain a photolabile group that can in turn be activated. As many rounds of photoactivation and nucleotide addition are performed as are required to synthesize oligonucleotides of the desired length.

This is repeated up to 60 times until the required sequences (up to 100.000) have been synthesized. Thus, the maximum length of any oligonucleotide produced on this platform is 60mer in length.

The microarray is now ready to be used, alternatively the oligonucleotides can be cleaved off to produce FleXelect oligopools.

From Wikipedia’s article on FlexGen B.V., shared under CC BY-SA 4.0.4

Timeline

  1. 2004Founded Founded1

Sources

The numbers on this page point here. Each one links to the record it came from.

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